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Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm, the Golgi apparatus & vesicles.
Antibody dilution
1:200
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA041622
Antibody HPA046754
Antibody HPA064827
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human gallbladder shows strong membranous positivity in glandular cells.
Expression
RNA: detected in 35 tissues Protein: detected in 38 cell types
RNA: detected in 35 tissues Protein: detected in 68 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:1500
1:125
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA041622
Antibody HPA046754
Antibody HPA064827
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
62, 38.7
62
62, 38.7
Antibody dilution
1:250
1:200
1:680
PROTEIN ARRAY
Antibody HPA041622
Antibody HPA046754
Antibody HPA064827
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.