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Immunohistochemical staining of human hippocampus shows moderate membranous and cytoplasmic positivity in fraction of neuronal cells. Neuropil was distinctly stained.
Immunohistochemical staining of human salivary gland shows strong cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 4 tissues Protein: detected in 7 cell types
RNA: detected in 4 tissues Protein: detected in 27 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:200
1:150
Literature conformity
Consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA035202
Antibody CAB001965
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Figure description
Target mass (kDa)
102.9, 101.5, 93.9, 92.2
102.9, 101.5, 93.9, 92.2
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA035202
Antibody CAB001965
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
