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Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol, centrosome & vesicles.
Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.
Antibody dilution
1:200
1:10
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA024705
Antibody HPA024781
Formal validation: Independent
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Pearson correlation with HPA024781 across 45 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA024705 across 45 cell lines.
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.
Expression
RNA: detected in 37 tissues Protein: detected in 72 cell types
RNA: detected in 37 tissues Protein: detected in 69 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:475
1:30
Literature conformity
Consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA024705
Antibody HPA024781
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY407583)
Target mass (kDa)
44.3, 41.5, 19.6
44.3, 41.5, 19.6
Antibody dilution
1:250
1:125
PROTEIN ARRAY
Antibody HPA024705
Antibody HPA024781
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
