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MCU

ANTIBODY INFORMATION
 
 

Antibody HPA016480

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA016480  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 4  

References

 11  

VALIDATION SUMMARY
 
 

Antibody HPA016480

  
Immunocytochemistry

Supported  
Immunohistochemistry

Approved  
Western blot

Supported  
Protein array

Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA016480

  

Standard validation

 Supported  

 

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.  

Antibody dilution

 1:4  

Literature conformity

 The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA016480

  

Standard validation

 Approved  

 

Figure description

 Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 79 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:7  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA016480

  

Standard validation

 Supported

Single band corresponding to the predicted size in kDa (+/-20%).		  

 

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY408629)		  

Target mass (kDa)

 39.9, 37, 35.2  

Antibody dilution

 1:125  

PROTEIN ARRAY
 
 

Antibody HPA016480

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA016480

  

Antigen

 Recombinant protein fragment  

Length (aa)

 106  

Antigen sequence

 
HHRTVHQRIASWQNLGAVYCSTVVPSDDVTVVYQNGLPVISVRLPSRRER
CQFTLKPISDSVGVFLRQLQEEDRGIDRVAIYSPDGVRVAASTGIDLLLL
DDFKLV
 

Matching transcripts

 MCU-001 - ENSP00000349680 [100%]
MCU-002 - ENSP00000362144 [100%]
MCU-201 - ENSP00000440913 [96%]
 

Other gene match

  
ANTIGEN VIEW
MCU-001
MCU-002
MCU-201

RELEVANT PUBLICATIONS
 
 

Antibody HPA016480

  

1.

 Integrative genomics identifies MCU as an essential component of the mitochondrial calcium uniporter
Baughman JM et al
Nature 2011;476(7360):341-5
  

2.

 The physiological role of mitochondrial calcium revealed by mice lacking the mitochondrial calcium uniporter
Pan X et al
Nat Cell Biol 2013;15(12):1464-72
  

3.

 Mitochondrial calcium uniporter Mcu controls excitotoxicity and is transcriptionally repressed by neuroprotective nuclear calcium signals
Qiu J et al
Nat Commun 2013;4:2034
  

4.

 Loss-of-function mutations in MICU1 cause a brain and muscle disorder linked to primary alterations in mitochondrial calcium signaling
Logan CV et al
Nat Genet 2014;46(2):188-93
  

5.

 Reconstitution of the mitochondrial calcium uniporter in yeast
Kovács-Bogdán E et al
Proc Natl Acad Sci U S A 2014;111(24):8985-90
  

6.

 Chronic enrichment of hepatic endoplasmic reticulum-mitochondria contact leads to mitochondrial dysfunction in obesity
Arruda AP et al
Nat Med 2014;20(12):1427-35
Application: WB 		 

7.

 Quantitative proteomics of synaptic and nonsynaptic mitochondria: insights for synaptic mitochondrial vulnerability
Stauch KL et al
J Proteome Res 2014;13(5):2620-36
Application: WB 		 

8.

 Structure and function of the N-terminal domain of the human mitochondrial calcium uniporter
Lee Y et al
EMBO Rep 2015;16(10):1318-33
Application: WB 		 

9.

 The endoplasmic reticulum mitochondrial calcium cross talk is downregulated in malignant pleural mesothelioma cells and plays a critical role in apoptosis inhibition
Patergnani S et al
Oncotarget 2015;6(27):23427-44
Application: IHC, WB 		 

10.

 Essential role of mitochondrial Ca2+ uniporter in the generation of mitochondrial pH gradient and metabolism-secretion coupling in insulin-releasing cells
Quan X et al
J Biol Chem 2015;290(7):4086-96
Application: WB 		 

11.

 The mitochondrial calcium uniporter regulates breast cancer progression via HIF-1α
Tosatto A et al
EMBO Mol Med 2016;8(5):569-85
Application: WB 		 
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.