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Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
Antibody dilution
1:128
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA034773
Antibody HPA034774
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human small intestine shows cytoplasmic positivity of endothelial cells.
Expression
RNA: detected in 33 tissues Protein: detected in 49 cell types
RNA: detected in 33 tissues Protein: detected in 44 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:50
1:300
Literature conformity
Partly consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA034773
Antibody HPA034774
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
188.7, 187.2
188.7, 187.2
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA034773
Antibody HPA034774
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
