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Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
Antibody dilution
1:65
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA038118
Antibody HPA042122
Antibody HPA042770
Standard validation
Approved
Approved
Approved
Figure description
Immunohistochemical staining of human cerebellum shows strong nuclear and cytoplasmic positivity in purkinje cells.
Immunohistochemical staining of human cerebral cortex shows distinct cytoplasmic and nuclear positivity in neuronal cells.
Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Expression
RNA: detected in 34 tissues Protein: detected in 42 cell types
RNA: detected in 34 tissues Protein: detected in 58 cell types
RNA: detected in 34 tissues Protein: detected in 33 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:30
1:300
1:125
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly consistent with RNA expression data.
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA038118
Antibody HPA042122
Antibody HPA042770
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
