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HKDC1

ANTIBODY INFORMATION
 
 

Antibody HPA011956

  

Antibody HPA064198

  

Antibody HPA064230

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA011956   HPA064198   HPA064230  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

 HK1 - ENSG00000156515 [80%]
      

Released in version

 4   14   14  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA011956

  

Antibody HPA064198

  

Antibody HPA064230

  
Immunocytochemistry

 

Validated
  

Validated
  
Immunohistochemistry

Approved      
Western blot

Supported   Uncertain   Uncertain  
Protein array

Supported   Supported   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA011956

  

Antibody HPA064198

  

Antibody HPA064230

  

Formal validation: Independent

  
Validated

Antibody staining overlaps with antibody HPA064230.		  
Validated

Antibody staining overlaps with antibody HPA064198.		  
 

Standard validation

   Supported   Supported  

     

Figure description

   Immunofluorescent staining of human cell line CACO-2 shows localization to mitochondria.   Immunofluorescent staining of human cell line A549 shows localization to mitochondria.  

Antibody dilution

   1:81   1:154  

Literature conformity

   The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA011956

  

Antibody HPA064198

  

Antibody HPA064230

  

Standard validation

 Approved      

     

Figure description

 Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in granular pattern in neuronal cells.      

Expression

 RNA: detected in 16 tissues
Protein: detected in 57 cell types		      

Retrieval

 HIER pH6      

Antibody dilution

 1:150      

Literature conformity

 No avaliable gene/protein characterization data.      

RNA consistency

 Mainly consistent with RNA expression data.      

WESTERN BLOT
 
 

Antibody HPA011956

  

Antibody HPA064198

  

Antibody HPA064230

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		      

Target mass (kDa)

 102.7, 102.5, 102.2, 101.1   102.5   102.5  

Antibody dilution

 1:250   1:270   1:520  

PROTEIN ARRAY
 
 

Antibody HPA011956

  

Antibody HPA064198

  

Antibody HPA064230

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3250   1:6200  

ANTIGEN INFORMATION
 
 

Antibody HPA011956

  

Antibody HPA064198

  

Antibody HPA064230

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 94   64   63  

Antigen sequence

 
QGTGEELFDHIVQCIADFLDYMGLKGASLPLGFTFSFPCRQMSIDKGTLI
GWTKGFKATDCEGEDVVDMLREAIKRRNEFDLDIVAVVNDTVGT
  
DLGGSKFRVLKVQVAEEGKRHVQMESQFYPTPNEIIRGNGTELFEYVADC
LADFMKTKDLKHKK
  
VQAQRKQIDRVLALFQLTREQLVDVQAKMRAELEYGLKKKSHGLATVRML
PTYVCGLPDGTEK
 

Matching transcripts

 HKDC1-001 - ENSP00000346643 [100%]
  HKDC1-001 - ENSP00000346643 [100%]
  HKDC1-001 - ENSP00000346643 [100%]
 

Other gene match

 HK1 - ENSG00000156515 [80%]
      
ANTIGEN VIEW
HKDC1-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.