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ANTIBODY INFORMATION
 
 

Antibody HPA007043

  

Antibody HPA007044

  

Antibody HPA011956

  

Antibody CAB010052

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA007043   HPA007044   HPA011956   sc-46695  

Host species

 Rabbit   Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

     HKDC1 - ENSG00000156510 [100%]
    

Released in version

 5   3   4   3  

References

 1   1      

VALIDATION SUMMARY
 
 

Antibody HPA007043

  

Antibody HPA007044

  

Antibody HPA011956

  

Antibody CAB010052

  
Immunocytochemistry

  Supported     Supported  
Immunohistochemistry


Validated
  

Validated
   Supported   Supported  
Western blot

Supported   Supported   Supported   Supported  
Protein array

Supported   Approved   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA007043

  

Antibody HPA007044

  

Antibody HPA011956

  

Antibody CAB010052

  

Standard validation

   Supported     Supported  

       

Figure description

   Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.     Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.  

Antibody dilution

   1:5     1:150  

Literature conformity

   The subcellular location is supported by literature.     The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA007043

  

Antibody HPA007044

  

Antibody HPA011956

  

Antibody CAB010052

  

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		      

 

  
 

      

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA007044 across 75 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA007043 across 75 cell types.		      
 

Standard validation

 Supported   Supported   Supported   Supported  

       

Figure description

 Immunohistochemical staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.   Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in parietal cells.   Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in granular pattern in neuronal cells.   Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 60 cell types		   RNA: detected in 37 tissues
Protein: detected in 61 cell types		   RNA: detected in 37 tissues
Protein: detected in 57 cell types		   RNA: detected in 37 tissues
Protein: detected in 67 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:50   1:15   1:150   1:600  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA007043

  

Antibody HPA007044

  

Antibody HPA011956

  

Antibody CAB010052

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

       

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 102.7, 102.5, 102.2, 101.1   102.7, 102.5, 102.2, 101.1, 32.7   102.7, 102.5, 102.2, 101.1   102.7, 102.5, 102.2, 101.1, 32.7, 15.7, 13  

Antibody dilution

 1:250   1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA007043

  

Antibody HPA007044

  

Antibody HPA011956

  

Antibody CAB010052

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:500   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA007043

  

Antibody HPA007044

  

Antibody HPA011956

  

Antibody CAB010052

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 136   127   94    

Antigen sequence

 
VSGMYLGELVRLILVKMAKEGLLFEGRITPELLTRGKFNTSDVSAIEKNK
EGLHNAKEILTRLGVEPSDDDCVSVQHVCTIVSFRSANLVAATLGAILNR
LRDNKGTPRLRTTVGVDGSLYKTHPQYSRRFHKTLR
  
LPTFVRSIPDGSEKGDFIALDLGGSSFRILRVQVNHEKNQNVHMESEVYD
TPENIVHGSGSQLFDHVAECLGDFMEKRKIKDKKLPVGFTFSFPCQQSKI
DEAILITWTKRFKASGVEGADVVKLLN
  
QGTGEELFDHIVQCIADFLDYMGLKGASLPLGFTFSFPCRQMSIDKGTLI
GWTKGFKATDCEGEDVVDMLREAIKRRNEFDLDIVAVVNDTVGT
  

											 
																				

																		

										
											
Matching transcripts

												
																					HK1-001 - ENSP00000352398 [100%] 
HK1-201 - ENSP00000298649 [100%] 
HK1-202 - ENSP00000353433 [100%] 
HK1-203 - ENSP00000402103 [100%] 

											 
																						HK1-001 - ENSP00000352398 [100%] 
HK1-016 - ENSP00000415949 [100%] 
HK1-201 - ENSP00000298649 [100%] 
HK1-202 - ENSP00000353433 [100%] 
HK1-203 - ENSP00000402103 [100%] 

											 
																						HK1-001 - ENSP00000352398 [80%] 
HK1-201 - ENSP00000298649 [80%] 
HK1-202 - ENSP00000353433 [80%] 
HK1-203 - ENSP00000402103 [80%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						HKDC1 - ENSG00000156510 [100%]

											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
HK1-001
HK1-016
HK1-201
HK1-202
HK1-203
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA007043

												
										 
																				
											
											
											
Antibody HPA007044

												
										 
																				
											
											
											
Antibody HPA011956

												
										 
																				
											
											
											
Antibody CAB010052

												
										 
																		

																	

										
											
1.

												
																						In vivo imaging of therapy response to a novel pan-HER antibody mixture using FDG and FLT positron emission tomography

	Nielsen CH et al

	Oncotarget 2015;6(35):37486-99

			Application: IHC				
											 
																							Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy

	Stadler C et al

	J Proteomics 2012;75(7):2236-51

			
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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