MS4A1 - Antibodies - The Human Protein Atlas

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MS4A1

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA014341

Antibody HPA014391

Antibody CAB000015

Provider

Atlas Antibodies
Sigma-Aldrich

DakoCytomation

Product name

HPA014341

HPA014391

M0755

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Supernatant

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA014341

Antibody HPA014391

Antibody CAB000015

Immunohistochemistry

Western blot

Supported

Uncertain

Protein array

Supported

IMMUNOHISTOCHEMISTRY

Antibody HPA014341

Antibody HPA014391

Antibody CAB000015

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.

Formal validation: Independent

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Pearson correlation with HPA014391 across 46 cell lines.

Distribution of protein expression (antibody staining).
Pearson correlation with HPA014341 across 46 cell lines.

Standard validation

Supported

Figure description

Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells of white pulp.

Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in lymphoid cells.

Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells in white pulp.

Expression

RNA: detected in 28 tissues
Protein: detected in 11 cell types

RNA: detected in 28 tissues
Protein: detected in 24 cell types

RNA: detected in 28 tissues
Protein: detected in 9 cell types

Retrieval

HIER pH6

HIER pH9

Antibody dilution

1:400

1:25

1:1000

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Consistent with RNA expression data.

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA014341

Antibody HPA014391

Antibody CAB000015

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

33.1, 31.6, 14.6

33.1, 31.6

33.1, 31.6, 14.6, 13.7, 0.8, 0.7

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA014341

Antibody HPA014391

Antibody CAB000015

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:500

ANTIGEN INFORMATION

Antibody HPA014341

Antibody HPA014391

Antibody CAB000015

Antigen

Recombinant protein fragment

Native protein

Length (aa)

Antigen sequence

ENEWKRTCSRPKSNIVLLSAEEKKEQTIEIKEEVVGLTETSSQPKNEEDI
EIIPIQEEEEEETETNFPEPPQDQESSP

MESLNFIRAHTPYINIYNCEPANPSEKNSPSTQYCY

Matching transcripts

MS4A1-001 - ENSP00000433277 [100%]
MS4A1-002 - ENSP00000433519 [100%]
MS4A1-003 - ENSP00000432270 [100%]
MS4A1-201 - ENSP00000314620 [100%]
MS4A1-202 - ENSP00000374589 [100%]

MS4A1-001 - ENSP00000433277 [100%]
MS4A1-002 - ENSP00000433519 [100%]
MS4A1-201 - ENSP00000314620 [100%]
MS4A1-202 - ENSP00000374589 [100%]

Other gene match

ANTIGEN VIEW

MS4A1-001	MS4A1-002	MS4A1-003	MS4A1-201	MS4A1-202

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.