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Immunohistochemical staining of human stomach shows strong positivity in enteroendocrine cells.
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in islet cells.
Expression
RNA: detected in 21 tissues Protein: detected in 12 cell types
RNA: detected in 21 tissues Protein: detected in 7 cell types
Retrieval
HIER pH6
HIER pH9
Antibody dilution
1:700
1:4000
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
Standard validation
Supported
Figure description
Staining in subsets of neuronal cell bodies and dendrites was observed, as well as synaptic positivity in selected brain areas.
WESTERN BLOT
Antibody HPA019472
Antibody CAB000075
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY420765)
Target mass (kDa)
12.7
12.7
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA019472
Antibody CAB000075
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
