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SLC38A10

ANTIBODY INFORMATION
 
 

Antibody HPA021374

  

Antibody HPA023161

  

Antibody HPA024631

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA021374   HPA023161   HPA024631  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 5   5   5  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA021374

  

Antibody HPA023161

  

Antibody HPA024631

  
Immunocytochemistry


Validated
    

Validated
  
Immunohistochemistry


Validated
  

Validated
   Supported  
Western blot

Uncertain   Uncertain   Supported  
Protein array

Approved   Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA021374

  

Antibody HPA023161

  

Antibody HPA024631

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA024631.		    
Validated

Antibody staining overlaps with antibody HPA021374.		  
 

Standard validation

 Supported     Supported  

     

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.     Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.  

Antibody dilution

 1:100     1:50  

Literature conformity

 The subcellular location is supported by literature.     The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA021374

  

Antibody HPA023161

  

Antibody HPA024631

  

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		    

 

  
 

    

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA023161 across 74 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA021374 across 74 cell types.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human stomach shows strong cytoplasmic positivity with granular pattern.   Immunohistochemical staining of human placenta shows strong cytoplasmic and nuclear positivity in decidual cells.   Immunohistochemical staining of human prostate shows strong cytoplasmic positivity with a granular pattern in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 77 cell types		   RNA: detected in 37 tissues
Protein: detected in 73 cell types		   RNA: detected in 37 tissues
Protein: detected in 61 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:150   1:100   1:150  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA021374

  

Antibody HPA023161

  

Antibody HPA024631

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

     Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 119.8, 83.3   119.8   119.8, 83.3  

Antibody dilution

 1:250   1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA021374

  

Antibody HPA023161

  

Antibody HPA024631

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA021374

  

Antibody HPA023161

  

Antibody HPA024631

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 89   63   90  

Antigen sequence

 
PVPHDKVVVDEGQDREVPEENKPPSRHAGGKAPGVQGQMAPPLPDSEREK
QEPEQGEVGKRPGQAQALEEAGDLPEDPQKVPEADGQPA
  
MKPKQVSRDLGLAADLPGGAEGAAAQPQAVLRQPELRVISDGEQGGQQGH
RLDHGGHLEMRKA
  
PEDLAGEAPGGRLGEAEGLMKVEAARLSAQDPVVAVAEDGREKPKLPKER
EELEQAQIKGPVDVPGREDGKEAPEEAQLDRPGQGIAVPV
 

Matching transcripts

 SLC38A10-001 - ENSP00000363891 [100%]
SLC38A10-002 - ENSP00000288439 [100%]
  SLC38A10-001 - ENSP00000363891 [100%]
  SLC38A10-001 - ENSP00000363891 [99%]
SLC38A10-002 - ENSP00000288439 [99%]
 

Other gene match

      
ANTIGEN VIEW
SLC38A10-001
SLC38A10-002
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.