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Immunofluorescent staining of human cell line Hep G2 shows localization to the Golgi apparatus.
Antibody dilution
1:29
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA065257
Antibody HPA066762
Antibody HPA069281
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in renal tubules.
Immunohistochemical staining of human colon shows distinct membranous and cytoplasmic positivity in glandular cells while weak cytoplasmic staining in peripheral nerve/ganglion.
Expression
RNA: detected in 36 tissues Protein: detected in 44 cell types
RNA: detected in 36 tissues Protein: detected in 42 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:1500
1:25
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA065257
Antibody HPA066762
Antibody HPA069281
Standard validation
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
68.6, 54.5, 16.2, 9.2
68.6, 54.5, 52.1, 40.6, 19.9
68.6, 16.2
Antibody dilution
1:250
1:90
1:100
PROTEIN ARRAY
Antibody HPA065257
Antibody HPA066762
Antibody HPA069281
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
