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Antibody staining overlaps with antibody HPA068374.
Validated
Antibody staining overlaps with antibody HPA051296.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear speckles.
Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear speckles.
Antibody dilution
1:24
1:41
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA051296
Antibody HPA068374
Antibody CAB025876
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human cerebral cortex shows strong positivity with a granular patter in neurons.
Expression
RNA: detected in 27 tissues Protein: detected in 51 cell types
Retrieval
HIER pH6
Antibody dilution
1:10
Literature conformity
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA051296
Antibody HPA068374
Antibody CAB025876
Standard validation
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
90.2, 36
90.2
90.2, 36
Antibody dilution
1:120
1:140
1:500
PROTEIN ARRAY
Antibody HPA051296
Antibody HPA068374
Antibody CAB025876
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
