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TFF2

ANTIBODY INFORMATION
 
 

Antibody HPA036705

  

Antibody CAB025834

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   R&D Systems
  

Product name

 HPA036705   MAB4077  

Host species

 Rabbit   Mouse  

Clonality

 pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

    

Released in version

 12   6  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA036705

  

Antibody CAB025834

  
Immunohistochemistry

Supported  

Validated
  
Western blot

Uncertain   Uncertain  
Protein array

Approved    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA036705

  

Antibody CAB025834

  

Formal validation: Orthogonal

  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  

 
 

 

  

Figure description

   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 12 tissues
Protein: detected in 7 cell types		   RNA: detected in 12 tissues
Protein: detected in 5 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:900   1:500  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA036705

  

Antibody CAB025834

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 14.3   14.3  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA036705

  

Antibody CAB025834

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA036705

  

Antibody CAB025834

  

Antigen

 Recombinant protein fragment   Recombinant protein  

Length (aa)

 83    

Antigen sequence

 
PHNRTNCGFPGITSDQCFDNGCCFDSSVTGVPWCFHPLPKQESDQCVMEV
SDRRNCGYPGISPEECASRKCCFSNFIFEVPWC
   

Matching transcripts

 TFF2-001 - ENSP00000291526 [100%]
   

Other gene match

    
ANTIGEN VIEW
TFF2-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.