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Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human stomach shows strong cytoplasmic and membranous positivity in glandular cells.
Expression
RNA: detected in 18 tissues Protein: detected in 6 cell types
RNA: detected in 18 tissues Protein: detected in 22 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:40000
1:200
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA003425
Antibody CAB002170
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY418822)
Target mass (kDa)
9.1
9.1
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA003425
Antibody CAB002170
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:3000
ANTIGEN INFORMATION
Antibody HPA003425
Antibody CAB002170
Antigen
Recombinant protein fragment
Not known
Length (aa)
53
Antigen sequence
QTETCTVAPRERQNCGFPGVTPSQCANKGCCFDDTVRGVPWCFYPNTIDV
PPE
