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Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
Antibody dilution
1:11
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA017381
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human rectum shows strong positivity in enteroendocrine cells.
Expression
RNA: detected in 36 tissues Protein: detected in 20 cell types
Retrieval
HIER pH6
Antibody dilution
1:400
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA017381
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY403257)
Target mass (kDa)
45.5
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA017381
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:500
ANTIGEN INFORMATION
Antibody HPA017381
Antigen
Recombinant protein fragment
Length (aa)
102
Antigen sequence
AGSVGLALCGQTLVVRGGSRFLATSIASSDDDSLFIYDCSAAEKKSQENK
GEDAPLDQGSGAILASTFSNSGSYFALTDDSKRLILFRTKPWQCLSVRTV
AR
