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Antibody staining overlaps with antibody HPA018166.
Validated
Antibody staining overlaps with antibody HPA016818.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line A-431 shows localization to nucleoli.
Immunofluorescent staining of human cell line A-431 shows localization to nucleoli.
Antibody dilution
1:61
1:40
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA016818
Antibody HPA018166
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human cerebral cortex shows strong nuclear(nucleolar) positivity in neuronal cells.
Immunohistochemical staining of human hippocampus shows nucleolar positivity in neuronal cells.
Expression
RNA: detected in 37 tissues Protein: detected in 28 cell types
RNA: detected in 37 tissues Protein: detected in 21 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:275
1:275
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA016818
Antibody HPA018166
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY418501)
Target mass (kDa)
52.8
52.8
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA016818
Antibody HPA018166
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
