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FTCD

ANTIBODY INFORMATION
 
 

Antibody HPA020073

  

Antibody HPA030928

  

Antibody HPA030929

  

Antibody HPA036114

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA020073   HPA030928   HPA030929   HPA036114  

Host species

 Rabbit   Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

        

Released in version

 5   14   12   13  

References

        

VALIDATION SUMMARY
 
 

Antibody HPA020073

  

Antibody HPA030928

  

Antibody HPA030929

  

Antibody HPA036114

  
Immunocytochemistry

   

Validated
  

Validated
  
Immunohistochemistry

Supported   Supported   Supported  

Validated
  
Western blot

Supported   Supported   Supported   Supported  
Protein array

Supported   Supported   Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA020073

  

Antibody HPA030928

  

Antibody HPA030929

  

Antibody HPA036114

  

Formal validation: Independent

    
Validated

Antibody staining overlaps with antibody HPA036114.		  
Validated

Antibody staining overlaps with antibody HPA030929.		  
 

Standard validation

     Approved   Approved  

       

Figure description

     Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.   Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.  

Antibody dilution

     1:93   1:133  

Literature conformity

     The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA020073

  

Antibody HPA030928

  

Antibody HPA030929

  

Antibody HPA036114

  

Formal validation: Orthogonal

      
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  

     
 

 

  

Figure description

       Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.  
 

Standard validation

 Supported   Supported   Supported   Supported  

       

Figure description

 Immunohistochemical staining of human liver shows cytoplasmic positivity in hepatocytes.   Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.   Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.   Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.  

Expression

 RNA: detected in 6 tissues
Protein: detected in 39 cell types		   RNA: detected in 6 tissues
Protein: detected in 11 cell types		   RNA: detected in 6 tissues
Protein: detected in 4 cell types		   RNA: detected in 6 tissues
Protein: detected in 7 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:10   1:75   1:1500   1:2000  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA020073

  

Antibody HPA030928

  

Antibody HPA030929

  

Antibody HPA036114

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

       

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 61.3, 58.9   61.3, 58.9, 52.8   61.3, 58.9, 52.8   61.3, 58.9, 52.8  

Antibody dilution

 1:250   1:250   1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA020073

  

Antibody HPA030928

  

Antibody HPA030929

  

Antibody HPA036114

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:500   1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA020073

  

Antibody HPA030928

  

Antibody HPA030929

  

Antibody HPA036114

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 96   89   82   70  

Antigen sequence

 
MRLPKNIPEEKDRRTAALQEGLRRAVSVPLTLAETVASLWLALQELARCG
NLACRSDLQVAAKALEMGVFGAYFNVLINLRDITDEAFKDQIHHRV
  
TKEQAHRIALNLREQGRGKDQPGRLKKVQGIGWYLDEKNLAQVSTNLLDF
EVTALHTVYEETCREAQELSLPVVGSQLVGLVPLKALLD
  
MSQLVECVPNFSEGKNQEVIDAISGAITQTPGCVLLDVDAGPSTNRTVYT
FVGPPECVVEGALNAARVASRLIDMSRHQGEH
  
GVSVDECVLCAQAFGQRLAEELDVPVYLYGEAARMDSRRTLPAIRAGEYE
ALPKKLQQADWAPDFGPSSF
 

Matching transcripts

 FTCD-001 - ENSP00000380854 [98%]
FTCD-004 - ENSP00000291670 [98%]
FTCD-005 - ENSP00000380856 [93%]
  FTCD-001 - ENSP00000380854 [100%]
FTCD-002 - ENSP00000380851 [100%]
FTCD-004 - ENSP00000291670 [100%]
FTCD-005 - ENSP00000380856 [100%]
  FTCD-001 - ENSP00000380854 [100%]
FTCD-002 - ENSP00000380851 [100%]
FTCD-004 - ENSP00000291670 [100%]
FTCD-005 - ENSP00000380856 [100%]
  FTCD-001 - ENSP00000380854 [100%]
FTCD-002 - ENSP00000380851 [100%]
FTCD-004 - ENSP00000291670 [100%]
FTCD-005 - ENSP00000380856 [100%]
 

Other gene match

        
ANTIGEN VIEW
FTCD-001
FTCD-002
FTCD-004
FTCD-005
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.