We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Immunohistochemical staining of human cerebral cortex shows strong nuclear and cytoplasmic positivity in glial cells.
Immunohistochemical staining of human cerebral cortex shows strong nuclear and cytoplasmic positivity in glial cells and strong cytoplasmic positivity in neuropil.
Immunohistochemical staining of human cerebral cortex shows cytoplasmic and nuclear positivity in neuronal and glial cells.
Expression
RNA: detected in 34 tissues Protein: detected in 46 cell types
RNA: detected in 34 tissues Protein: detected in 31 cell types
RNA: detected in 34 tissues Protein: detected in 18 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:5000
1:5000
1:1500
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA015768
Antibody CAB000073
Antibody CAB078196
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY416735)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
10.7
10.7, 10.3
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA015768
Antibody CAB000073
Antibody CAB078196
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
