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Immunohistochemical staining of human kidney shows strong luminal membranous positivity in tubules.
Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.
Expression
RNA: detected in 14 tissues Protein: detected in 29 cell types
RNA: detected in 14 tissues Protein: detected in 40 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:100
1:30000
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA042272
Antibody CAB046014
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402605)
Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
54.5, 48
54.5, 48, 22.7, 15.4
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA042272
Antibody CAB046014
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
