RECQL4 - Antibodies - The Human Protein Atlas

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RECQL4

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA025821

Antibody CAB017800

Provider

Atlas Antibodies
Sigma-Aldrich

SDIX

Product name

HPA025821

2547.00.02

Host species

Rabbit

Clonality

pAb

msAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Affinity

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA025821

Antibody CAB017800

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA025821

Antibody CAB017800

Formal validation: Genetic

Validated

Significant downregulation > 25% by both siRNA:s.

Relative fluorescence intensity (RFI)

Figure description

Nuclear region of segmented cells in 10x-images

Antibody dilution

1:40

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.

Antibody dilution

1:50

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA025821

Antibody CAB017800

Standard validation

Supported

Figure description

Immunohistochemical staining of human thyroid gland shows distinct nuclear positivity in glandular cells.

Immunohistochemical staining of human duodenum shows strong nuclear and slightly weaker cytoplasmic positivity in glandular cells.

Expression

RNA: detected in 33 tissues
Protein: detected in 64 cell types

RNA: detected in 33 tissues
Protein: detected in 78 cell types

Retrieval

HIER pH6

Antibody dilution

1:25

1:50

Literature conformity

Partly consistent with extensive gene/protein characterization data.

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

Mainly not consistent with RNA expression data.

WESTERN BLOT

Antibody HPA025821

Antibody CAB017800

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

133.1

133.1, 94.6, 42.8, 38.3, 33.3, 24.9

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA025821

Antibody CAB017800

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA025821

Antibody CAB017800

Antigen

Recombinant protein fragment

Genetic immunization

Length (aa)

112

Antigen sequence

WLQRCHSEVPDFLGAPKACRPDLGSEESQLLIPGESAVLGPGAGSQGPEA
SAFQEVSIRVGSPQPSSSGGEKRRWNEEPWESPAQVQQESSQAGPPSEGA
GAVAVEEDPPGE

Matching transcripts

RECQL4-002 - ENSP00000482313 [100%]

Other gene match

ANTIGEN VIEW

RECQL4-002

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.