Fields »
ABOUT
HELP
BLOG
CELF5

ANTIBODY INFORMATION
 
 

Antibody HPA042012

  

Antibody HPA060336

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA042012   HPA060336  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 8   12  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA042012

  

Antibody HPA060336

  
Immunohistochemistry

Supported   Supported  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA042012

  

Antibody HPA060336

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.   Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.  

Expression

 RNA: detected in 4 tissues
Protein: detected in 20 cell types		   RNA: detected in 4 tissues
Protein: detected in 4 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:50   1:250  

Literature conformity

 Consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA042012

  

Antibody HPA060336

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

   

Figure description

    

Target mass (kDa)

 52.4   52.4, 44.5  

Antibody dilution

 1:250   1:230  

PROTEIN ARRAY
 
 

Antibody HPA042012

  

Antibody HPA060336

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:2750  

ANTIGEN INFORMATION
 
 

Antibody HPA042012

  

Antibody HPA060336

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 70   47  

Antigen sequence

 
AFSPCHIQQIGAVSLNGLPATPIAPASGLHSPPLLGTTAVPGLVAPITNG
FAGVVPFPGGHPALETVYAN
  
AQSPTVAETLHPAFSGVQQYTAMYPTAAITPIAHSVPQPPPLLQQQQ
 

Matching transcripts

 CELF5-001 - ENSP00000292672 [100%]
  CELF5-001 - ENSP00000292672 [100%]
CELF5-002 - ENSP00000443498 [100%]
 

Other gene match

    
ANTIGEN VIEW
CELF5-001
CELF5-002
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.