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HFM1

ANTIBODY INFORMATION
 
 

Antibody HPA035036

  

Antibody HPA035557

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA035036   HPA035557  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 13   7  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA035036

  

Antibody HPA035557

  
Immunocytochemistry

Approved   Approved  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA035036

  

Antibody HPA035557

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunofluorescent staining of human cell line AF22 shows localization to the Golgi apparatus.   Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.  

Antibody dilution

 1:98   1:200  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.  

WESTERN BLOT
 
 

Antibody HPA035036

  

Antibody HPA035557

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

   

Figure description

    

Target mass (kDa)

 162.6, 70.9   162.6, 70.9  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA035036

  

Antibody HPA035557

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA035036

  

Antibody HPA035557

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 87   68  

Antigen sequence

 
SSVPPVKRLKIQMNKSQSVDLKEFGFTPKPSLPSISRSEYLNISELPIME
QWDQPEIYGKVRQEPSEYQDKEVLNVNFELGNEVWDD
  
FVGLDIQQKLTVFYLEPKRFGNQITMQRKSETQISHSKHSDISTIAGPNK
GTTASKKPGNRECNHLCK
 

Matching transcripts

 HFM1-002 - ENSP00000359454 [100%]
HFM1-008 - ENSP00000387661 [100%]
  HFM1-002 - ENSP00000359454 [100%]
HFM1-008 - ENSP00000387661 [100%]
 

Other gene match

    
ANTIGEN VIEW
HFM1-002
HFM1-008
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.