VCAM1 - Antibodies - The Human Protein Atlas

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VCAM1

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA034796

Antibody HPA069867

Antibody CAB000154

Provider

Atlas Antibodies
Sigma-Aldrich

DakoCytomation

Product name

HPA034796

HPA069867

M7106

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Supernatant

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA034796

Antibody HPA069867

Antibody CAB000154

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA034796

Antibody HPA069867

Antibody CAB000154

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to cell junctions.

Antibody dilution

1:85

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA034796

Antibody HPA069867

Antibody CAB000154

Standard validation

Supported

Figure description

Immunohistochemical staining of human tonsil shows moderate positivity in a subset of cells in germinal centers.

Immunohistochemical staining of human spleen shows strong positivity in cells in white pulp.

Expression

RNA: detected in 36 tissues
Protein: detected in 29 cell types

RNA: detected in 36 tissues
Protein: detected in 8 cell types

Retrieval

HIER pH6

Antibody dilution

1:150

1:50

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

Consistent with RNA expression data.

WESTERN BLOT

Antibody HPA034796

Antibody HPA069867

Antibody CAB000154

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

81.3, 74.3

81.3, 74.3, 71.3, 59.2

Antibody dilution

1:250

1:280

PROTEIN ARRAY

Antibody HPA034796

Antibody HPA069867

Antibody CAB000154

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:3400

ANTIGEN INFORMATION

Antibody HPA034796

Antibody HPA069867

Antibody CAB000154

Antigen

Recombinant protein fragment

Not known

Length (aa)

Antigen sequence

IGDSVMLTCSVMGCESPSFSWRTQIDSPLSGKVRSEGTNSTLTLSPVSFE
NEHSYLCTVTCGHKKLEKGIQVELYSFPRDPEIEMSG

ITVKCSVADVYPFDRLEIDLLKGDHLMKSQEFLEDADRKSLETKSLEVTF
TPVIEDIGKVLVCRAKLHIDEMDSVPT

Matching transcripts

VCAM1-001 - ENSP00000294728 [100%]
VCAM1-004 - ENSP00000359137 [100%]

VCAM1-001 - ENSP00000294728 [100%]
VCAM1-002 - ENSP00000304611 [100%]
VCAM1-003 - ENSP00000359133 [100%]
VCAM1-004 - ENSP00000359137 [100%]

Other gene match

ANTIGEN VIEW

VCAM1-001	VCAM1-002	VCAM1-003	VCAM1-004

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.