Fields »
ABOUT
HELP
BLOG
B3GALNT2

ANTIBODY INFORMATION
 
 

Antibody HPA012502

  

Antibody HPA050662

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA012502   HPA050662  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 5   14  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA012502

  

Antibody HPA050662

  
Immunocytochemistry

Uncertain    
Immunohistochemistry

  Approved  
Western blot

Supported   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA012502

  

Antibody HPA050662

  

Standard validation

 Uncertain    

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & the Golgi apparatus.    

Antibody dilution

 1:9    

Literature conformity

 The subcellular location is not consistent with literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA012502

  

Antibody HPA050662

  

Standard validation

   Approved  

   

Figure description

   Immunohistochemical staining of human esophagus shows cytoplasmic positivity in squamous epithelial cells.  

Expression

   RNA: detected in 36 tissues
Protein: detected in 68 cell types		  

Retrieval

   HIER pH6  

Antibody dilution

   1:30  

Literature conformity

   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA012502

  

Antibody HPA050662

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 56.7, 35.6   56.7  

Antibody dilution

 1:250   1:220  

PROTEIN ARRAY
 
 

Antibody HPA012502

  

Antibody HPA050662

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:1800  

ANTIGEN INFORMATION
 
 

Antibody HPA012502

  

Antibody HPA050662

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 129   100  

Antigen sequence

 
PQWKSTHYDVVVGVLSARNNHELRNVIRSTWMRHLLQHPTLSQRVLVKFI
IGAHGCEVPVEDREDPYSCKLLNITNPVLNQEIEAFSLSEDTSSGLPEDR
VVSVSFRVLYPIVITSLGVFYDANDVGFQ
  
WTVETTSFNLLLKTDDDCYIDLEAVFNRIVQKNLDGPNFWWGNFRLNWAV
DRTGKWQELEYPSPAYPAFACGSGYVISKDIVKWLASNSGRLKTYQGEDV
 

Matching transcripts

 B3GALNT2-001 - ENSP00000355559 [100%]
B3GALNT2-002 - ENSP00000315678 [100%]
  B3GALNT2-001 - ENSP00000355559 [100%]
 

Other gene match

    
ANTIGEN VIEW
B3GALNT2-001
B3GALNT2-002
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.