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ALPP

ANTIBODY INFORMATION
 
 

Antibody HPA038764

  

Antibody HPA038765

  

Antibody HPA051699

  

Antibody CAB026327

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Lab Vision/NeoMarkers
  

Product name

 HPA038764   HPA038765   HPA051699   MS-734  

Host species

 Rabbit   Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

 ALPPL2 - ENSG00000163286 [100%]
ALPI - ENSG00000163295 [97%]
   ALPPL2 - ENSG00000163286 [91%]
ALPI - ENSG00000163295 [100%]
   ALPPL2 - ENSG00000163286 [100%]
ALPI - ENSG00000163295 [100%]
    

Released in version

 12   12   12   12  

References

        

VALIDATION SUMMARY
 
 

Antibody HPA038764

  

Antibody HPA038765

  

Antibody HPA051699

  

Antibody CAB026327

  
Immunocytochemistry

Supported        
Immunohistochemistry

Supported   Supported   Supported  

Validated
  
Western blot

Uncertain   Uncertain   Uncertain   Uncertain  
Protein array

Supported   Approved   Approved    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA038764

  

Antibody HPA038765

  

Antibody HPA051699

  

Antibody CAB026327

  

Standard validation

 Supported        

       

Figure description

 Immunofluorescent staining of human cell line HeLa shows localization to plasma membrane.        

Antibody dilution

 1:45        

Literature conformity

 The subcellular location is supported by literature.        

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA038764

  

Antibody HPA038765

  

Antibody HPA051699

  

Antibody CAB026327

  

Formal validation: Orthogonal

      
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

     
 

 

  

Figure description

       Distribution of protein expression (antibody staining) and RNA expression (TPM) across 41 cell lines.  
 

Standard validation

 Supported   Supported   Supported   Supported  

       

Figure description

 Immunohistochemical staining of human placenta shows strong cytoplasmic and membranous staining in trophoblasts.   Immunohistochemical staining of human placenta shows strong membranous and cytoplasmic positivity in trophoblastic cells.   Immunohistochemical staining of human placenta shows strong membranous positivity in trophoblastic cells.   Immunohistochemical staining of human placenta shows strong cytoplasmic and membranous positivity in trophoblastic cells.  

Expression

 RNA: detected in 4 tissues
Protein: detected in 5 cell types		   RNA: detected in 4 tissues
Protein: detected in 8 cell types		   RNA: detected in 4 tissues
Protein: detected in 9 cell types		   RNA: detected in 4 tissues
Protein: detected in 8 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:85   1:150   1:50   1:200  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA038764

  

Antibody HPA038765

  

Antibody HPA051699

  

Antibody CAB026327

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

       

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		     Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 58, 57.4, 57.3, 56.8   58, 57.4, 57.3, 56.8   58, 57.4, 57.3, 56.8   58  

Antibody dilution

 1:250   1:250   1:90   1:500  

PROTEIN ARRAY
 
 

Antibody HPA038764

  

Antibody HPA038765

  

Antibody HPA051699

  

Antibody CAB026327

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		    

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:3000   1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA038764

  

Antibody HPA038765

  

Antibody HPA051699

  

Antibody CAB026327

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Native protein  

Length (aa)

 30   33   31    

Antigen sequence

 
NWYSDADVPASARQEGCQDIATQLISNMDI
  
HRDPTLDPSLMEMTEAALRLLSRNPRGFYLFVE
  
FQTIGLSAAARFNQCNTTRGNEVISVMNRAK
  

											 
																				

																		

										
											
Matching transcripts

												
																					ALPP-001 - ENSP00000375881 [100%] 

											 
																						ALPP-001 - ENSP00000375881 [94%] 

											 
																						ALPP-001 - ENSP00000375881 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					ALPPL2 - ENSG00000163286 [100%]
ALPI - ENSG00000163295 [97%]

											 
																						ALPPL2 - ENSG00000163286 [91%]
ALPI - ENSG00000163295 [100%]

											 
																						ALPPL2 - ENSG00000163286 [100%]
ALPI - ENSG00000163295 [100%]

											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
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