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IGFN1

ANTIBODY INFORMATION
 
 

Antibody HPA050415

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA050415  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 12  

References

  

VALIDATION SUMMARY
 
 

Antibody HPA050415

  
Immunocytochemistry

Approved  
Immunohistochemistry

Supported  
Western blot

Uncertain  
Protein array

Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA050415

  

Standard validation

 Approved  

 

Figure description

 Immunofluorescent staining of human cell line HeLa shows localization to midbody ring.  

Antibody dilution

 1:175  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA050415

  

Standard validation

 Supported  

 

Figure description

 Immunohistochemical staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.  

Expression

 RNA: detected in 9 tissues
Protein: detected in 5 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:1200  

Literature conformity

 Consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA050415

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

 

Figure description

  

Target mass (kDa)

 383.8, 137.8, 119.6  

Antibody dilution

 1:100  

PROTEIN ARRAY
 
 

Antibody HPA050415

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:25000  

ANTIGEN INFORMATION
 
 

Antibody HPA050415

  

Antigen

 Recombinant protein fragment  

Length (aa)

 87  

Antigen sequence

 
AHSFRIRVAACPQAPGPIHLQENVPGTVTAEWEPSPDEAQDVPLHYAVFT
RSSAHGPWHEAADRIYTNRFTLLGILPGHEYHFRVVA
 

Matching transcripts

 IGFN1-001 - ENSP00000387975 [99%]
IGFN1-201 - ENSP00000295591 [99%]
IGFN1-202 - ENSP00000334714 [99%]
 

Other gene match

  
ANTIGEN VIEW
IGFN1-001
IGFN1-201
IGFN1-202
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.