ARPC2 - Antibodies - The Human Protein Atlas

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ARPC2

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA008352

Antibody CAB001994

Provider

Atlas Antibodies
Sigma-Aldrich

Upstate

Product name

HPA008352

07-227

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Not known

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA008352

Antibody CAB001994

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA008352

Antibody CAB001994

Standard validation

Uncertain

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to cytosol & the Golgi apparatus.

Antibody dilution

1:27

Literature conformity

The subcellular location is not consistent with literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA008352

Antibody CAB001994

Formal validation: Orthogonal

Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.

Standard validation

Approved

Figure description

Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in reaction center cells.

Immunohistochemical staining of human heart muscle shows moderate nuclear positivity in myocytes.

Expression

RNA: detected in 37 tissues
Protein: detected in 81 cell types

RNA: detected in 37 tissues
Protein: detected in 57 cell types

Retrieval

HIER pH6

Antibody dilution

1:300

1:750

Literature conformity

Partly consistent with gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA008352

Antibody CAB001994

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 229, 107, 78, 46, 31, 26, 16.2
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

34.3

34.3, 18.7, 10

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA008352

Antibody CAB001994

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA008352

Antibody CAB001994

Antigen

Recombinant protein fragment

Not known

Length (aa)

139

Antigen sequence

TVVFSTVFKDDDDVVIGKVFMQEFKEGRRASHTAPQVLFSHREPPLELKD
TDAAVGDNIGYITFVLFPRHTNASARDNTINLIHTFRDYLHYHIKCSKAY
IHTRMRAKTSDFLKVLNRARPDAEKKEMKTITGKTFSSR

Matching transcripts

ARPC2-001 - ENSP00000295685 [100%]
ARPC2-002 - ENSP00000327137 [100%]

Other gene match

ANTIGEN VIEW

ARPC2-001	ARPC2-002

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.