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Immunohistochemical staining of human tonsil shows cytoplasmic positivity in lymphoid cells outside the reaction centra.
Immunohistochemical staining of human lymph node shows cytoplasmic positivity in a subsets of lymphoid cells.
Expression
RNA: detected in 16 tissues Protein: detected in 4 cell types
RNA: detected in 16 tissues Protein: detected in 27 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:100
1:100
Literature conformity
Consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA002356
Antibody CAB026130
Standard validation
Uncertain
Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY413992)
Target mass (kDa)
21.2, 17.1
21.2, 17.1
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA002356
Antibody CAB026130
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
