SUCLG1 - Antibodies - The Human Protein Atlas

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SUCLG1

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA036683

Antibody HPA036684

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA036683

HPA036684

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA036683

Antibody HPA036684

Immunocytochemistry

Supported

Immunohistochemistry

Western blot

Supported

Protein array

Approved

Supported

IMMUNOCYTOCHEMISTRY

Antibody HPA036683

Antibody HPA036684

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & mitochondria.

Antibody dilution

1:116

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA036683

Antibody HPA036684

Formal validation: Independent

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Pearson correlation with HPA036684 across 46 cell lines.

Distribution of protein expression (antibody staining).
Pearson correlation with HPA036683 across 46 cell lines.

Formal validation: Independent

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Spearman correlation with HPA036684 across 76 cell types.

Distribution of protein expression (antibody staining).
Spearman correlation with HPA036683 across 76 cell types.

Standard validation

Supported

Figure description

Immunohistochemical staining of human colon shows strong cytoplasmic positivity(granular pattern) in glandular cells.

Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in renal tubules.

Expression

RNA: detected in 37 tissues
Protein: detected in 81 cell types

RNA: detected in 37 tissues
Protein: detected in 72 cell types

Retrieval

HIER pH6

Antibody dilution

1:1200

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Consistent with RNA expression data.

WESTERN BLOT

Antibody HPA036683

Antibody HPA036684

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

36.2

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA036683

Antibody HPA036684

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA036683

Antibody HPA036684

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

LPQNGIRHCSYTASRQHLYVDKNTKIICQGFTGKQGTFHSQQALEYGTKL
VGGTTPGKGGQTHLGLPVFNTVKEAKEQTGA

ASVIYVPPPFAAAAINEAIEAEIPLVVCITEGIPQQDMVRVKHKLLRQEK
TRLIGPNCPGVINPGECKIGIM

Matching transcripts

SUCLG1-001 - ENSP00000377446 [100%]

Other gene match

ANTIGEN VIEW

SUCLG1-001

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.