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Immunofluorescent staining of human cell line RT4 shows localization to plasma membrane.
Antibody dilution
1:103
Literature conformity
The subcellular location is not consistent with literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA065290
Antibody CAB002213
Standard validation
Approved
Figure description
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
Expression
RNA: detected in 32 tissues Protein: detected in 56 cell types
Retrieval
HIER pH6
Antibody dilution
1:10
Literature conformity
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA065290
Antibody CAB002213
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Target too small/large to be analyzed with the present setup.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
277.5, 276.9, 274.9, 255.9
277.5, 276.9, 274.9, 255.9, 25, 15.3
Antibody dilution
1:340
1:500
PROTEIN ARRAY
Antibody HPA065290
Antibody CAB002213
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.