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APBB2

ANTIBODY INFORMATION
 
 

Antibody HPA023542

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA023542  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 5  

References

  

VALIDATION SUMMARY
 
 

Antibody HPA023542

  
Immunocytochemistry

Approved  
Immunohistochemistry

Uncertain  
Western blot


Validated
  
Protein array

Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA023542

  

Standard validation

 Approved  

 

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.  

Antibody dilution

 1:100  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA023542

  

Standard validation

 Uncertain  

 

Figure description

 Immunohistochemical staining of human pancreas shows distinct granular positivity in exocrine cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 72 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:1500  

Literature conformity

 No avaliable gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA023542

  

Formal validation: Genetic
Validated

Downregulation visible in both siRNA lanes		  

 

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled		  

Target mass (kDa)

 83.4, 81, 80.9, 80  

Loading control

 Total protein image  

Antibody dilution

 1:256  
 

Standard validation

 Supported

Band of predicted size in kDa (+/-20%) with additional bands present.		  

 

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY406673)		  

Target mass (kDa)

 83.4, 81, 80.9, 80  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA023542

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA023542

  

Antigen

 Recombinant protein fragment  

Length (aa)

 125  

Antigen sequence

 
SEKLEGKEPHPQDSSSCEILPSQPRRTKSFLNYYADLETSARELEQNRGN
HHGTAEEKSQPVQGQASTIIGNGDLLLQKPNRPQSSPEDGQVATVSSSPE
TKKDHPKTGAKTDCALHRIQNLAPS
 

Matching transcripts

 APBB2-001 - ENSP00000426018 [100%]
APBB2-002 - ENSP00000295974 [100%]
APBB2-003 - ENSP00000427211 [100%]
APBB2-006 - ENSP00000427307 [100%]
APBB2-012 - ENSP00000421539 [100%]
 

Other gene match

  
ANTIGEN VIEW
APBB2-001
APBB2-002
APBB2-003
APBB2-006
APBB2-012
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.