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Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.
Antibody dilution
1:100
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA030497
Standard validation
Approved
Figure description
Immunohistochemical staining of human testis shows distinct cytoplasmic positivity occasionally combined with nuclear staining in seminiferous duct cells.
Expression
RNA: detected in 37 tissues Protein: detected in 11 cell types
Retrieval
HIER pH6
Antibody dilution
1:350
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA030497
Standard validation
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
39.4, 37.5
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA030497
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
