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TOPBP1

ANTIBODY INFORMATION
 
 

Antibody HPA036738

  

Antibody HPA036739

  

Antibody CAB022451

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Chemicon
  

Product name

 HPA036738   HPA036739   AB3245  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

      

Released in version

 13   12   5  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA036738

  

Antibody HPA036739

  

Antibody CAB022451

  
Immunocytochemistry

Supported   Supported    
Immunohistochemistry

    Approved  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Supported   Approved    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA036738

  

Antibody HPA036739

  

Antibody CAB022451

  

Standard validation

 Supported   Supported    

     

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nucleus & nuclear bodies.   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus.    

Antibody dilution

 1:81   1:159    

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA036738

  

Antibody HPA036739

  

Antibody CAB022451

  

Standard validation

     Approved  

     

Figure description

     Immunohistochemical staining of human duodenum shows strong nuclear positivity in glandular cells.  

Expression

     RNA: detected in 37 tissues
Protein: detected in 79 cell types		  

Retrieval

     HIER pH6  

Antibody dilution

     1:75  

Literature conformity

     Consistent with extensive gene/protein characterization data.  

RNA consistency

     Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA036738

  

Antibody HPA036739

  

Antibody CAB022451

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		      

Target mass (kDa)

 170.7   170.7   170.7, 23.3  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA036738

  

Antibody HPA036739

  

Antibody CAB022451

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA036738

  

Antibody HPA036739

  

Antibody CAB022451

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Synthetic peptide  

Length (aa)

 93   94    

Antigen sequence

 
KYEAAKKWNLPAVTIAWLLETARTGKRADESHFLIENSTKEERSLETEIT
NGINLNSDTAEHPGTRLQTHRKTVVTPLDMNRFQSKAFRAVVS
  
TVPDVNTEPSQNEQIIWDDPTAREERARLASNLQWPSCPTQYSELQVDIQ
NLEDSPFQKPLHDSEIAKQAVCDPGNIRVTEAPKHPISEELETP
  

											 
																				

																		

										
											
Matching transcripts

												
																					TOPBP1-001 - ENSP00000260810 [100%] 

											 
																						TOPBP1-001 - ENSP00000260810 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
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