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SENP2

ANTIBODY INFORMATION
 
 

Antibody HPA029247

  

Antibody HPA029248

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA029247   HPA029248  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 6   6  

References

   1  

VALIDATION SUMMARY
 
 

Antibody HPA029247

  

Antibody HPA029248

  
Immunocytochemistry

  Supported  
Immunohistochemistry

Approved   Approved  
Western blot

Supported   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA029247

  

Antibody HPA029248

  

Standard validation

   Supported  

   

Figure description

   Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.  

Antibody dilution

   1:200  

Literature conformity

   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA029247

  

Antibody HPA029248

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 72 cell types		   RNA: detected in 37 tissues
Protein: detected in 64 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:75   1:350  

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA029247

  

Antibody HPA029248

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 67.9   67.9  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA029247

  

Antibody HPA029248

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA029247

  

Antibody HPA029248

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 86   84  

Antigen sequence

 
SGHGNSVCPVTSNYHSSQRSQMDTLKTKGWGEEQNHGVKTTQFVPKQYRL
VETRGPLCSLRSEKRCSKGKITDTETMVGIRFENES
  
RRGYQLEPDLSEEVSARLRLGSGSNGLLRRKVSIIETKEKNCSGKERDRR
TDDLLELTEDMEKEISNALGHGPQDEILSSAFKL
 

Matching transcripts

 SENP2-001 - ENSP00000296257 [100%]
  SENP2-001 - ENSP00000296257 [100%]
 

Other gene match

    
ANTIGEN VIEW
SENP2-001

RELEVANT PUBLICATIONS
 
 

Antibody HPA029247

  

Antibody HPA029248

  

1.

   Integrative genomics analysis identifies candidate drivers at 3q26-29 amplicon in squamous cell carcinoma of the lung
Wang J et al
Clin Cancer Res 2013;19(20):5580-90
  
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.