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RHO

ANTIBODY INFORMATION
 
 

Antibody HPA013440

  

Antibody CAB022486

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Chemicon
  

Product name

 HPA013440   MAB5316  

Host species

 Rabbit   Mouse  

Clonality

 pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

    

Released in version

 16   16  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA013440

  

Antibody CAB022486

  
Immunohistochemistry

Supported   Supported  
Western blot

Uncertain   Uncertain  
Protein array

Supported    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA013440

  

Antibody CAB022486

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human retina shows strong cytoplasmic positivity in rod segments.   Immunohistochemical staining of human retina shows strong cytoplasmic positivity in outer segments of photoreceptor cells.  

Expression

 RNA: detected in 0 tissues
Protein: detected in 1 cell types		   RNA: detected in 0 tissues
Protein: detected in 1 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:150   1:4000  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 No internal RNA expression data available for correlation.   No internal RNA expression data available for correlation.  

WESTERN BLOT
 
 

Antibody HPA013440

  

Antibody CAB022486

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

   

Figure description

    

Target mass (kDa)

 38.9   38.9  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA013440

  

Antibody CAB022486

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA013440

  

Antibody CAB022486

  

Antigen

 Recombinant protein fragment   Native protein  

Length (aa)

 38    

Antigen sequence

 
MNGTEGPNFYVPFSNATGVVRSPFEYPQYYLAEPWQFS
   

Matching transcripts

 RHO-001 - ENSP00000296271 [100%]
   

Other gene match

    
ANTIGEN VIEW
RHO-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.