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ATRIP

ANTIBODY INFORMATION
 
 

Antibody HPA047590

  

Antibody CAB033109

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA047590   sc-33410  

Host species

 Rabbit   Goat  

Clonality

 pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

 ATRIP - ENSG00000282827 [80%]
    

Released in version

 13   6  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA047590

  

Antibody CAB033109

  
Immunocytochemistry

Approved    
Immunohistochemistry

  Approved  
Western blot

Supported   Uncertain  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA047590

  

Antibody CAB033109

  

Standard validation

 Approved    

   

Figure description

 Immunofluorescent staining of human cell line HeLa shows localization to nucleus.    

Antibody dilution

 1:104    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA047590

  

Antibody CAB033109

  

Standard validation

   Approved  

   

Figure description

   Immunohistochemical staining of human prostate shows strong nuclear positivity in glandular cells.  

Expression

   RNA: detected in 36 tissues
Protein: detected in 73 cell types		  

Retrieval

   HIER pH6  

Antibody dilution

   1:10  

Literature conformity

   Consistent with extensive gene/protein characterization data.  

RNA consistency

   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA047590

  

Antibody CAB033109

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 85.8, 83, 76.3, 72.4, 22.4   85.8, 83, 76.3, 22.4  

Antibody dilution

 1:520   1:500  

PROTEIN ARRAY
 
 

Antibody HPA047590

  

Antibody CAB033109

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:4150    

ANTIGEN INFORMATION
 
 

Antibody HPA047590

  

Antibody CAB033109

  

Antigen

 Recombinant protein fragment   Synthetic peptide  

Length (aa)

 123    

Antigen sequence

 
RETVPIKDNFELEVLQAQYKELKEKMKVMEEEVLIKNGEIKILRDSLHQT
ESVLEEQRRSHFLLEQEKTQALSDKEKEFSKKLQSLQSELQFKDAEMNEL
RTKLQTSERANKLAAPSVSHVSP
  

											 
																				

																		

										
											
Matching transcripts

												
																					ATRIP-001 - ENSP00000323099 [100%] 
ATRIP-002 - ENSP00000302338 [100%] 
ATRIP-005 - ENSP00000400930 [100%] 
ATRIP-007 - ENSP00000489608 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					ATRIP - ENSG00000282827 [80%]

											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
ATRIP-001
ATRIP-002
ATRIP-005
ATRIP-007
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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