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HPGD

ANTIBODY INFORMATION
 
 

Antibody HPA004919

  

Antibody HPA005679

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA004919   HPA005679  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 3   3  

References

 2    

VALIDATION SUMMARY
 
 

Antibody HPA004919

  

Antibody HPA005679

  
Immunocytochemistry

  Supported  
Immunohistochemistry


Validated

Validated
  

Validated

Validated
  
Western blot

Supported   Supported  
Protein array

Approved   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA004919

  

Antibody HPA005679

  

Standard validation

   Supported  

   

Figure description

   Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.  

Antibody dilution

   1:70  

Literature conformity

   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA004919

  

Antibody HPA005679

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 

 

  
 

 

  

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  

 

  
 

  

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA005679 across 46 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA004919 across 46 cell lines.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human stomach shows strong cytoplasmic and membranous positivity in glandular cells.   Immunohistochemical staining of human urinary bladder shows strong cytoplasmic and nuclear positivity in urothelial cells.  

Expression

 RNA: detected in 32 tissues
Protein: detected in 55 cell types		   RNA: detected in 32 tissues
Protein: detected in 31 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:100   1:250  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA004919

  

Antibody HPA005679

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

 Lane 1: Marker [kDa] 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 29, 19.3, 19.1, 15.5   29, 21.5, 15.7, 10.7, 10.5  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA004919

  

Antibody HPA005679

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:1000  

ANTIGEN INFORMATION
 
 

Antibody HPA004919

  

Antibody HPA005679

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 116   53  

Antigen sequence

 
VDWNLEAGVQCKAALDEQFEPQKTLFIQCDVADQQQLRDTFRKVVDHFGR
LDILVNNAGVNNEKNWEKTLQINLVSVISGTYLGLDYMSKQNGGEGGIII
NMSSLAGLMPVAQQPV
  
LAANLMNSGVRLNAICPGFVNTAILESIEKEENMGQYIEYKDHIKDMIKY
YGI
 

Matching transcripts

 HPGD-001 - ENSP00000296522 [100%]
HPGD-013 - ENSP00000296521 [100%]
HPGD-004 - ENSP00000420892 [93%]
HPGD-202 - ENSP00000443644 [91%]
  HPGD-001 - ENSP00000296522 [100%]
HPGD-002 - ENSP00000422418 [100%]
HPGD-008 - ENSP00000423066 [100%]
HPGD-011 - ENSP00000398720 [100%]
HPGD-201 - ENSP00000438017 [100%]
HPGD-010 - ENSP00000423110 [98%]
 

Other gene match

    
ANTIGEN VIEW
HPGD-001
HPGD-002
HPGD-004
HPGD-008
HPGD-010
HPGD-011
HPGD-013
HPGD-201
HPGD-202

RELEVANT PUBLICATIONS
 
 

Antibody HPA004919

  

Antibody HPA005679

  

1.

 Candidate serological biomarkers for cancer identified from the secretomes of 23 cancer cell lines and the human protein atlas
Wu CC et al
Mol Cell Proteomics 2010;9(6):1100-17
    

2.

 Characterization of transcriptional changes in ERG rearrangement-positive prostate cancer identifies the regulation of metabolic sensors such as neuropeptide Y
Massoner P et al
PLoS One 2013;8(2):e55207
    
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.