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COG5

ANTIBODY INFORMATION
 
 

Antibody HPA020300

  

Antibody HPA041583

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA020300   HPA041583  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 5   13  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA020300

  

Antibody HPA041583

  
Immunocytochemistry


Validated
  

Validated
  
Immunohistochemistry

Uncertain    
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA020300

  

Antibody HPA041583

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA041583.		  
Validated

Antibody staining overlaps with antibody HPA020300.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.   Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.  

Antibody dilution

 1:50   1:24  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA020300

  

Antibody HPA041583

  

Standard validation

 Uncertain    

   

Figure description

 Immunohistochemical staining of human colon shows strong cytoplasmic positivity in granular pattern in glandular cells.    

Expression

 RNA: detected in 37 tissues
Protein: detected in 79 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:500    

Literature conformity

 Consistent with extensive gene/protein characterization data.    

RNA consistency

 Mainly consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA020300

  

Antibody HPA041583

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 94.9, 92.7, 90.8   94.9, 92.7  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA020300

  

Antibody HPA041583

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA020300

  

Antibody HPA041583

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 121   130  

Antigen sequence

 
SFWTNMEKLMDHIYAVCGQVQHLQKVLAKKRDPVSHICFIEEIVKDGQPE
IFYTFWNSVTQALSSQFHMATNSSMFLKQAFEGEYPKLLRLYNDLWKRLQ
QYSQHIQGNFNASGTTDLYVD
  
LAVGPFCRRVSDLGKSYRMLRSFRPLLFQASEHVASSPALGDVIPFSIII
QFLFTRAPAELKSPFQRAEWSHTRFSQWLDDHPSEKDRLLLIRGALEAYV
QSVRSREGKEFAPVYPIMVQLLQKAMSALQ
 

Matching transcripts

 COG5-001 - ENSP00000334703 [100%]
COG5-002 - ENSP00000377228 [100%]
COG5-003 - ENSP00000297135 [100%]
  COG5-001 - ENSP00000334703 [100%]
COG5-003 - ENSP00000297135 [100%]
 

Other gene match

    
ANTIGEN VIEW
COG5-001
COG5-002
COG5-003
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.