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Immunofluorescent staining of human cell line PC-3 shows localization to nucleoli & cytosol.
Antibody dilution
1:108
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA008890
Antibody HPA045034
Antibody CAB008373
Standard validation
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in a subset of seminiferus ducts.
Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.
Expression
RNA: detected in 35 tissues Protein: detected in 54 cell types
RNA: detected in 35 tissues Protein: detected in 39 cell types
RNA: detected in 35 tissues Protein: detected in 37 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:100
1:450
1:100
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
No internal RNA expression data available for correlation.
WESTERN BLOT
Antibody HPA008890
Antibody HPA045034
Antibody CAB008373
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
22, 21.1
22, 21.1, 12.9
22, 12.9
Antibody dilution
1:250
1:250
1:500
PROTEIN ARRAY
Antibody HPA008890
Antibody HPA045034
Antibody CAB008373
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
