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Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm & cytosol.
Antibody dilution
1:70
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA055599
Antibody HPA063472
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
Expression
RNA: detected in 34 tissues Protein: detected in 7 cell types
Retrieval
HIER pH6
Antibody dilution
1:150
Literature conformity
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA055599
Antibody HPA063472
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400425)
Target mass (kDa)
33.1, 32.6, 22.6, 20.4
33.1, 32.6
Antibody dilution
1:125
1:240
PROTEIN ARRAY
Antibody HPA055599
Antibody HPA063472
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
