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Immunofluorescent staining of human cell line A-431 shows localization to nucleoli & vesicles.
Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
Antibody dilution
1:22
1:50
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA018156
Antibody CAB000457
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human duodenum shows cytoplasmic positivity in glandular cells. Brunner's glands were also positive while Paneth cells were negative.
Immunohistochemical staining of human liver shows strong cytoplasmic positivity in granular pattern in hepatocytes.
Expression
RNA: detected in 37 tissues Protein: detected in 76 cell types
RNA: detected in 37 tissues Protein: detected in 29 cell types
Retrieval
HIER pH6
None
Antibody dilution
1:25
1:200
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA018156
Antibody CAB000457
Standard validation
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
