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NOS3

ANTIBODY INFORMATION
 
 

Antibody CAB002168

  

Provider

 Novocastra
  

Product name

 NCL-NOS-3  

Host species

 Mouse  

Clonality

 mAb  

Purity

 Not known  

Other gene match

  

Released in version

 1  

References

  

VALIDATION SUMMARY
 
 

Antibody CAB002168

  
Immunohistochemistry


Validated
  
Western blot

Uncertain  

IMMUNOHISTOCHEMISTRY
 
 

Antibody CAB002168

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

Cell line01,0002,0003,0004,0005,000Protein expression
 
SH-SY5YU-138 MGU-251 MGU-87 MGA-431HaCaTSK-MEL-30WM-115HELHL-60HMC-1K-562NB-4THP-1U-937DaudiHDLM-2Karpas-707MOLT-4REHRPMI-8226U-266/70U-266/84U-698A549SCLC-21HCACO-2CAPAN-2Hep G2AN3-CAEFO-21HeLaMCF7SiHaSK-BR-3HEK 293NTERA-2PC-3RT4RH-30U-2 OSU-2197TIMEBEWO01,0002,0003,0004,0005,000Protein expressionBrainSkinMyeloidLymphoidLungAbdominalBreast, female reproductive systemRenal, urinary, male reproductive systemSarcomaEndothelialMiscellaneous

Cell line010203040506070RNA expression
 
SH-SY5YU-138 MGU-251 MGU-87 MGA-431HaCaTSK-MEL-30WM-115HELHL-60HMC-1K-562NB-4THP-1U-937DaudiHDLM-2Karpas-707MOLT-4REHRPMI-8226U-266/70U-266/84U-698A549SCLC-21HCACO-2CAPAN-2Hep G2AN3-CAEFO-21HeLaMCF7SiHaSK-BR-3HEK 293NTERA-2PC-3RT4RH-30U-2 OSU-2197TIMEBEWO010203040506070RNA expressionBrainSkinMyeloidLymphoidLungAbdominalBreast, female reproductive systemRenal, urinary, male reproductive systemSarcomaEndothelialMiscellaneous

  

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Standard validation

 Supported  

 

Figure description

 Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in endothelial cells.  

Expression

 RNA: detected in 33 tissues
Protein: detected in 3 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:10  

Literature conformity

 Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody CAB002168

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

 

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 133.3, 111.1, 69, 67.9, 33.8  

Antibody dilution

 1:500  

ANTIGEN INFORMATION
 
 

Antibody CAB002168

  

Antigen

 Not known  

Length (aa)

  

Antigen sequence

  

Matching transcripts

  

Other gene match

  
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.