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PSIP1

ANTIBODY INFORMATION
 
 

Antibody HPA019697

  

Antibody CAB013718

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   SDIX
  

Product name

 HPA019697   2115.00.02  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

    

Released in version

 5   4  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA019697

  

Antibody CAB013718

  
Immunocytochemistry


Validated
    
Immunohistochemistry

Supported   Supported  
Western blot

Uncertain   Uncertain  
Protein array

Approved    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA019697

  

Antibody CAB013718

  

Formal validation: Genetic
Validated

Significant downregulation > 25% by one siRNA.		    

 

Relative fluorescence intensity (RFI)

    

Figure description

 Nuclear region of segmented cells in 10x-images    

Antibody dilution

 1:172    
 

Standard validation

 Supported    

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.    

Antibody dilution

 1:200    

Literature conformity

 The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA019697

  

Antibody CAB013718

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human cerebral cortex shows strong nuclear as well as cytoplasmic positivity in neuronal and glial cells.   Immunohistochemical staining of human cerebellum shows strong nuclear positivity in cerebellar tissue.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 81 cell types		   RNA: detected in 37 tissues
Protein: detected in 75 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:800   1:800  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA019697

  

Antibody CAB013718

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

    

Target mass (kDa)

 60.1, 37.7, 37.3   60.1, 37.7, 37.3, 7.8  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA019697

  

Antibody CAB013718

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA019697

  

Antibody CAB013718

  

Antigen

 Recombinant protein fragment   Genetic immunization  

Length (aa)

 84    

Antigen sequence

 
KQVETEEAGVVTTATASVNLKVSPKRGRPAATEVKIPKPRGRPKMVKQPC
PSESDIITEEDKSKKKGQEEKQPKKQPKKDEEGQ
  

											 
																				

																		

										
											
Matching transcripts

												
																					PSIP1-003 - ENSP00000370109 [100%] 
PSIP1-004 - ENSP00000370114 [100%] 
PSIP1-006 - ENSP00000370091 [100%] 
PSIP1-007 - ENSP00000370092 [100%] 
PSIP1-201 - ENSP00000380653 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
PSIP1-003
PSIP1-004
PSIP1-006
PSIP1-007
PSIP1-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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