We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & microtubule organizing center.
Antibody dilution
1:63
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA035117
Antibody HPA035118
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human testis shows distinct cytoplasmic positivity in subsets of cells in seminiferus duct cells.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in subsets of cells in seminiferus duct cells.
Expression
RNA: detected in 37 tissues Protein: detected in 54 cell types
RNA: detected in 37 tissues Protein: detected in 57 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:75
1:150
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
Standard validation
Approved
Figure description
Cytoplasmic staining in subsets of neurons, most abundant in interbrain and hindbrain.
WESTERN BLOT
Antibody HPA035117
Antibody HPA035118
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY410835)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY410835)
Target mass (kDa)
42.1, 36.1
42.1
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA035117
Antibody HPA035118
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
