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Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.
Antibody dilution
1:200
Literature conformity
The subcellular location is not consistent with literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA039272
Standard validation
Approved
Figure description
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells of seminiferus ducts.
Expression
RNA: detected in 12 tissues Protein: detected in 7 cell types
Retrieval
HIER pH6
Antibody dilution
1:1750
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA039272
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY408058)
Target mass (kDa)
46.4, 44
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA039272
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
