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ANTIBODY INFORMATION
 
 

Antibody CAB004420

  

Provider

 Santa Cruz Biotechnology
  

Product name

 sc-28356  

Host species

 Mouse  

Clonality

 mAb  

Purity

 Protein A/G  

Other gene match

  

Released in version

 16  

References

  

VALIDATION SUMMARY
 
 

Antibody CAB004420

  
Immunohistochemistry


Validated
  
Western blot

Uncertain  

IMMUNOHISTOCHEMISTRY
 
 

Antibody CAB004420

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 

 

  

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Standard validation

 Supported  

 

Figure description

 Immunohistochemical staining of human bone marrow shows nuclear positivity in hematopoietic cells.  

Expression

 RNA: detected in 5 tissues
Protein: detected in 2 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:300  

Literature conformity

 Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody CAB004420

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

 

Figure description

  

Target mass (kDa)

 37.5, 32.4  

Antibody dilution

 1:500  

ANTIGEN INFORMATION
 
 

Antibody CAB004420

  

Antigen

 Recombinant protein  

Length (aa)

  

Antigen sequence

 

											 
																				

																		

										
											
Matching transcripts

												
																					
											 
																				

																		

										
											
Other gene match

												
																					
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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						The Human Protein Atlas project is funded
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