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ANTIBODY INFORMATION
 
 

Antibody HPA008356

  

Antibody HPA008495

  

Antibody CAB002581

  

Antibody CAB018342

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Novocastra
   Santa Cruz Biotechnology
  

Product name

 HPA008356   HPA008495   NCL-RET   sc-167  

Host species

 Rabbit   Rabbit   Mouse   Rabbit  

Clonality

 pAb   pAb   mAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known   Affinity  

Other gene match

        

Released in version

 4   16   1   4  

References

 2        

VALIDATION SUMMARY
 
 

Antibody HPA008356

  

Antibody HPA008495

  

Antibody CAB002581

  

Antibody CAB018342

  
Immunocytochemistry

Supported        
Immunohistochemistry

Approved     Approved   Approved  
Western blot

Uncertain   Uncertain   Uncertain   Uncertain  
Protein array

Approved   Supported      

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA008356

  

Antibody HPA008495

  

Antibody CAB002581

  

Antibody CAB018342

  

Standard validation

 Supported        

       

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol & vesicles.        

Antibody dilution

 1:179        

Literature conformity

 The subcellular location is supported by literature.        

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA008356

  

Antibody HPA008495

  

Antibody CAB002581

  

Antibody CAB018342

  

Standard validation

 Approved     Approved   Approved  

       

Figure description

 Immunohistochemical staining of human adrenal gland shows cytoplasmic membranous positivity in glandular cells.     Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.   Immunohistochemical staining of human adrenal gland shows cytoplasmic and membranous positivity in glandular cells.  

Expression

 RNA: detected in 19 tissues
Protein: detected in 71 cell types		     RNA: detected in 19 tissues
Protein: detected in 32 cell types		   RNA: detected in 19 tissues
Protein: detected in 77 cell types		  

Retrieval

 HIER pH6     HIER pH6   HIER pH6  

Antibody dilution

 1:250     1:60   1:45  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.     Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.     Not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  
 

Standard validation

   Supported      

       

Figure description

   Staining in neuronal cell bodies, processes and synapses in cortical, striatal, hippocampal and cerebellar areas among others.      

WESTERN BLOT
 
 

Antibody HPA008356

  

Antibody HPA008495

  

Antibody CAB002581

  

Antibody CAB018342

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

       

Figure description

     Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 124.3, 119.8   124.3, 119.8   124.3, 119.8, 51.2, 19.4   124.3, 119.8, 51.2, 19.4  

Antibody dilution

 1:250   1:250   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA008356

  

Antibody HPA008495

  

Antibody CAB002581

  

Antibody CAB018342

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		      

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:3000   1:500      

ANTIGEN INFORMATION
 
 

Antibody HPA008356

  

Antibody HPA008495

  

Antibody CAB002581

  

Antibody CAB018342

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Not known   Synthetic peptide  

Length (aa)

 147   147      

Antigen sequence

 
NQVSVDAFKILEDPKWEFPRKNLVLGKTLGEGEFGKVVKATAFHLKGRAG
YTTVAVKMLKENASPSELRDLLSEFNVLKQVNHPHVIKLYGACSQDGPLL
LIVEYAKYGSLRGFLRESRKVGPGYLGSGGSRNSSSLDHPDERALTM
  
NQVSVDAFKILEDPKWEFPRKNLVLGKTLGEGEFGKVVKATAFHLKGRAG
YTTVAVKMLKENASPSELRDLLSEFNVLKQVNHPHVIKLYGACSQDGPLL
LIVEYAKYGSLRGFLRESRKVGPGYLGSGGSRNSSSLDHPDERALTM
  

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					RET-001 - ENSP00000347942 [100%] 
RET-002 - ENSP00000344798 [100%] 

											 
																						RET-001 - ENSP00000347942 [100%] 
RET-002 - ENSP00000344798 [100%] 

											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
RET-001
RET-002
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA008356

												
										 
																				
											
											
											
Antibody HPA008495

												
										 
																				
											
											
											
Antibody CAB002581

												
										 
																				
											
											
											
Antibody CAB018342

												
										 
																		

																	

										
											
1.

												
																						RET is a potential tumor suppressor gene in colorectal cancer

	Luo Y et al

	Oncogene 2013;32(16):2037-47

			
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
2.

												
																						Profiling post-centrifugation delay of serum and plasma with antibody bead arrays

	Qundos U et al

	J Proteomics 2013;95:46-54

			
											 
																						
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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