Fields »
ABOUT
HELP
BLOG
APBB1

ANTIBODY INFORMATION
 
 

Antibody HPA038521

  

Antibody HPA038522

  

Antibody CAB022104

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Upstate
  

Product name

 HPA038521   HPA038522   05-758  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

      

Released in version

 12   12   5  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA038521

  

Antibody HPA038522

  

Antibody CAB022104

  
Immunocytochemistry


Validated
  

Validated
    
Immunohistochemistry

Approved   Approved   Approved  
Western blot

Uncertain   Supported   Uncertain  
Protein array

Approved   Approved    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA038521

  

Antibody HPA038522

  

Antibody CAB022104

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA038522.		  
Validated

Antibody staining overlaps with antibody HPA038521.		    
 

Standard validation

 Supported   Supported    

     

Figure description

 Immunofluorescent staining of human cell line HEK 293 shows localization to plasma membrane.   Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.    

Antibody dilution

 1:114   1:67    

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA038521

  

Antibody HPA038522

  

Antibody CAB022104

  

Standard validation

 Approved   Approved   Approved  

     

Figure description

 Immunohistochemical staining of human cerebral cortex shows neuropil and cytoplasmic positivity in neurons.   Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules, cells in glomeruli were moderately stained.   Immunohistochemical staining of human lateral ventricle shows distinct cytoplasmic positivity in subsets of glial cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 60 cell types		   RNA: detected in 37 tissues
Protein: detected in 73 cell types		   RNA: detected in 37 tissues
Protein: detected in 49 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:300   1:300   1:35  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA038521

  

Antibody HPA038522

  

Antibody CAB022104

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

     

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 77.2, 77, 54.2, 53.9, 52.7, 52.6, 49.9, 49.8, 29.2   77.2, 77   77.2, 77, 54.2, 53.9, 52.7, 52.6, 49.9, 49.8, 29.2, 25.7, 9.7  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA038521

  

Antibody HPA038522

  

Antibody CAB022104

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:6000   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA038521

  

Antibody HPA038522

  

Antibody CAB022104

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 73   93    

Antigen sequence

 
QEESQLTWTGFAHGEGFEDGEFWKDEPSDEAPMELGLKEPEEGTLTFPAQ
SLSPEPLPQEEEKLPPRNTNPGI
  
PGPANAKWLKEGQNQLRRAATAHRDQNRNVTLTLAEEASQEPEMAPLGPK
GLIHLYSELELSAHNAANRGLRGPGLIISTQEQGPDEGEEKAA
  

											 
																				

																		

										
											
Matching transcripts

												
																					APBB1-001 - ENSP00000311912 [100%] 
APBB1-002 - ENSP00000299402 [100%] 
APBB1-004 - ENSP00000476536 [100%] 
APBB1-005 - ENSP00000476442 [100%] 
APBB1-007 - ENSP00000433338 [100%] 
APBB1-014 - ENSP00000476871 [100%] 
APBB1-018 - ENSP00000477069 [100%] 
APBB1-023 - ENSP00000477213 [100%] 
APBB1-024 - ENSP00000476846 [100%] 
APBB1-025 - ENSP00000476646 [100%] 
APBB1-201 - ENSP00000374556 [100%] 
APBB1-202 - ENSP00000483682 [100%] 
APBB1-203 - ENSP00000484493 [100%] 
APBB1-204 - ENSP00000481817 [100%] 

											 
																						APBB1-001 - ENSP00000311912 [100%] 
APBB1-002 - ENSP00000299402 [100%] 
APBB1-023 - ENSP00000477213 [100%] 
APBB1-201 - ENSP00000374556 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

					«					
						

							

								

									
APBB1-001
APBB1-002
APBB1-004
APBB1-005
APBB1-007
APBB1-014
APBB1-018
APBB1-023
APBB1-024
APBB1-025
APBB1-201
APBB1-202
APBB1-203
APBB1-204
								

							

						

							
					»				

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

					Contact
				

				
				

					
					
					
					
				

				
			


			

				

					The Project
				
			
				
			

			
			

			
				

					The Human Protein Atlas
				

				
			

			
			

			

						The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.