AP1G1 - Antibodies - The Human Protein Atlas

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AP1G1

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA041224

Antibody CAB009049

Provider

Atlas Antibodies
Sigma-Aldrich

Santa Cruz Biotechnology

Product name

HPA041224

sc-10763

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Protein A/G

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA041224

Antibody CAB009049

Immunocytochemistry

Immunohistochemistry

Approved

Western blot

Uncertain

Protein array

Supported

IMMUNOCYTOCHEMISTRY

Antibody HPA041224

Antibody CAB009049

Formal validation: Genetic

Validated

Significant downregulation > 25% by both siRNA:s.

Relative fluorescence intensity (RFI)

Figure description

Cytoplasmic region of segmented cells in 10x-images

Antibody dilution

1:28

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.

Antibody dilution

1:28

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA041224

Antibody CAB009049

Standard validation

Approved

Figure description

Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 74 cell types

Retrieval

HIER pH6

Antibody dilution

1:25

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA041224

Antibody CAB009049

Formal validation: Genetic

Validated

Downregulation visible in both siRNA lanes

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled

Target mass (kDa)

91.7, 91.4, 26.3

Loading control

Total protein image

Antibody dilution

1:61

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

91.7, 91.4, 26.3

91.7, 91.4, 26.3, 21.4, 16.6, 16, 10.8, 10, 6.3, 3.1

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA041224

Antibody CAB009049

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA041224

Antibody CAB009049

Antigen

Recombinant protein fragment

Recombinant protein

Length (aa)

Antigen sequence

FLLDGLSSQPLFNDIAAGIPSITAYSKNGLKIEFTFERSNTNPSVTVITI
QASNSTELDMTDFVFQAAVPKTFQLQLLSPSSSIVPAFNTGTI

Matching transcripts

AP1G1-001 - ENSP00000299980 [100%]
AP1G1-002 - ENSP00000454523 [100%]
AP1G1-008 - ENSP00000454219 [100%]
AP1G1-014 - ENSP00000377148 [100%]

Other gene match

ANTIGEN VIEW

AP1G1-001	AP1G1-002	AP1G1-008	AP1G1-014

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.